PCR-based reverse genetics strategy for bluetongue virus recovery

PCR-based reverse genetics strategy for bluetongue virus recovery

Abstract Background Bluetongue virus (BTV), an emerging insect vector mediated pathogen affecting both wild ruminants and livestock, has a genome consisting of 10 linear double-stranded RNA genome segments. BTV has a severe economic impact on agriculture in many parts of the world. Current reverse g...

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Bibliographic Details
Main Authors: Qingyuan Xu, Jinying Ge, Maolin Li, Encheng Sun, Yawei Zhou, Yunze Guo, Donglai Wu, Zhigao Bu
Format: Article
Language:English
Published: BMC 2019-12-01
Series:Virology Journal
Subjects:
Bluetongue virus
Reverse genetics
T7 RNA polymerase
Genome modification
Reassortment
Online Access:https://doi.org/10.1186/s12985-019-1261-2

Internet

https://doi.org/10.1186/s12985-019-1261-2

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