Continuous long-term cytotoxicity monitoring in 3D spheroids of beetle luciferase-expressing hepatocytes by nondestructive bioluminescence measurement

Continuous long-term cytotoxicity monitoring in 3D spheroids of beetle luciferase-expressing hepatocytes by nondestructive bioluminescence measurement

Abstract Background Three-dimensional (3D) spheroids are frequently used in toxicological study because their morphology and function closely resemble those of tissue. As these properties are maintained over a long term, repeated treatment of the spheroids with a test object is possible. Generally,...

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Bibliographic Details
Main Authors: Mayu Yasunaga, Yasuko Fujita, Rumiko Saito, Mitsuo Oshimura, Yoshihiro Nakajima
Format: Article
Language:English
Published: BMC 2017-06-01
Series:BMC Biotechnology
Subjects:
Luciferase
3D culture
Artificial chromosome vector
Long-term monitoring
Cytotoxicity
Emerald Luc (ELuc)
Online Access:http://link.springer.com/article/10.1186/s12896-017-0374-1

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http://link.springer.com/article/10.1186/s12896-017-0374-1

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