Reconstruction of Axial Tomographic High Resolution Data from Confocal Fluorescence Microscopy: A Method for Improving 3D FISH Images

Fluorescent confocal laser scanning microscopy allows an improved imaging of microscopic objects in three dimensions. However, the resolution along the axial direction is three times worse than the resolution in lateral directions. A method to overcome this axial limitation is tilting the object und...

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Bibliographic Details
Main Authors: R. Heintzmann, G. Kreth, C. Cremer
Format: Article
Language:English
Published: Hindawi Limited 2000-01-01
Series:Analytical Cellular Pathology
Online Access:http://dx.doi.org/10.1155/2000/459351