Reconstruction of Axial Tomographic High Resolution Data from Confocal Fluorescence Microscopy: A Method for Improving 3D FISH Images
Fluorescent confocal laser scanning microscopy allows an improved imaging of microscopic objects in three dimensions. However, the resolution along the axial direction is three times worse than the resolution in lateral directions. A method to overcome this axial limitation is tilting the object und...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2000-01-01
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Series: | Analytical Cellular Pathology |
Online Access: | http://dx.doi.org/10.1155/2000/459351 |