Multiplex, DNase-free one-step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte-specific mRNAs

Multiplex, DNase-free one-step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte-specific mRNAs

Abstract Background Plasmodium gametocytes are sexual stages transmitted to female Anopheles mosquitoes. While Plasmodium parasites can be differentiated microscopically on Giemsa-stained blood smears, molecular methods are increasingly used because of their increased sensitivity. Molecular detectio...

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Bibliographic Details
Main Authors: Amelia E. Hanron, Zachary P. Billman, Annette M. Seilie, Tayla M. Olsen, Matthew Fishbaugher, Ming Chang, Thomas Rueckle, Nicole Andenmatten, Bryan Greenhouse, Emmanuel Arinaitwe, John Rek, Smita Das, Gonzalo J. Domingo, Kelly Shipman, Stefan H. Kappe, James G. Kublin, Sean C. Murphy
Format: Article
Language:English
Published: BMC 2017-05-01
Series:Malaria Journal
Subjects:
Gametocyte
mRNA
Spliced
Antisense
Plasmodium
DNase
Online Access:http://link.springer.com/article/10.1186/s12936-017-1863-3

Internet

http://link.springer.com/article/10.1186/s12936-017-1863-3

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