In vitro cultivation and immunostaining of Lawsonia intracellularis strains

In vitro cultivation and immunostaining of Lawsonia intracellularis strains

The aim of the study was to implement in vitro cultivation of L. intracellularis strains using ATCC 55783 and vaccine strains, and McCoy cells (ATCC CRL-1696). The infection was monitored by daily observations under phase contrast microscope. Indirect immunostaining using monoclonal antibody was als...

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Bibliographic Details
Main Authors: Szczotka-Bochniarz Anna, Podgórska Katarzyna, Nowak Agnieszka, Pejsak Zygmunt
Format: Article
Language:English
Published: Sciendo 2013-09-01
Series:Bulletin of the Veterinary Institute in Pulawy
Subjects:
pigs
lawsonia intracellularis
in vitro cultivation
mccoy cells
immunostaining
Online Access:https://doi.org/10.2478/bvip-2013-0055

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