In vitro cultivation and immunostaining of Lawsonia intracellularis strains
The aim of the study was to implement in vitro cultivation of L. intracellularis strains using ATCC 55783 and vaccine strains, and McCoy cells (ATCC CRL-1696). The infection was monitored by daily observations under phase contrast microscope. Indirect immunostaining using monoclonal antibody was als...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Sciendo
2013-09-01
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Series: | Bulletin of the Veterinary Institute in Pulawy |
Subjects: | |
Online Access: | https://doi.org/10.2478/bvip-2013-0055 |