Random sampling causes the low reproducibility of rare eukaryotic OTUs in Illumina COI metabarcoding

DNA metabarcoding, the PCR-based profiling of natural communities, is becoming the method of choice for biodiversity monitoring because it circumvents some of the limitations inherent to traditional ecological surveys. However, potential sources of bias that can affect the reproducibility of this me...

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Bibliographic Details
Main Authors: Matthieu Leray, Nancy Knowlton
Format: Article
Language:English
Published: PeerJ Inc. 2017-03-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/3006.pdf