Designing, optimization, and validation of whole blood direct T-ARMS PCR for precise and rapid genotyping of complex vertebral malformation in cattle

Designing, optimization, and validation of whole blood direct T-ARMS PCR for precise and rapid genotyping of complex vertebral malformation in cattle

Abstract Background DNA testing in the cattle industry undergoes multiple hurdles. Successful genotyping involves the transportation of samples from the field to the laboratory in a chilled environment followed by DNA extraction, and finally, a specific genotyping protocol is followed. Various resea...

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Bibliographic Details
Main Authors: R. R. Alyethodi, U. Singh, S. Kumar, R. Alex, G. S. Sengar, T. V. Raja, R. Deb, B. Prakash
Format: Article
Language:English
Published: BMC 2021-05-01
Series:BMC Biotechnology
Subjects:
T-ARMS PCR
Genetic testing
Direct T-ARMS PCR
Blood
Heparin
EDTA
Online Access:https://doi.org/10.1186/s12896-021-00696-5

Internet

https://doi.org/10.1186/s12896-021-00696-5

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