Determining Trap Compliances, Microsphere Size Variations, and Response Linearities in Single DNA Molecule Elasticity Measurements with Optical Tweezers

Determining Trap Compliances, Microsphere Size Variations, and Response Linearities in Single DNA Molecule Elasticity Measurements with Optical Tweezers

We previously introduced the use of DNA molecules for calibration of biophysical force and displacement measurements with optical tweezers. Force and length scale factors can be determined from measurements of DNA stretching. Trap compliance can be determined by fitting the data to a nonlinear DNA e...

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Bibliographic Details
Main Authors: Youbin Mo, Mounir Fizari, Kristina Koharchik, Douglas E. Smith
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-03-01
Series:Frontiers in Molecular Biosciences
Subjects:
optical trap
laser tweezers
single-molecule
DNA elasticity
calibration
microsphere size
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2021.605102/full

Internet

https://www.frontiersin.org/articles/10.3389/fmolb.2021.605102/full

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