High frequency targeted mutagenesis using engineered endonucleases and DNA-end processing enzymes.

High frequency targeted mutagenesis using engineered endonucleases and DNA-end processing enzymes.

Targeting DNA double-strand breaks is a powerful strategy for gene inactivation applications. Without the use of a repair plasmid, targeted mutagenesis can be achieved through Non-Homologous End joining (NHEJ) pathways. However, many of the DNA breaks produced by engineered nucleases may be subject...

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Bibliographic Details
Main Authors: Fabien Delacôte, Christophe Perez, Valérie Guyot, Marianne Duhamel, Christelle Rochon, Nathalie Ollivier, Rachel Macmaster, George H Silva, Frédéric Pâques, Fayza Daboussi, Philippe Duchateau
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23359797/pdf/?tool=EBI

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https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23359797/pdf/?tool=EBI

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