Cut-and-Paste of DNA Using an Artificial Restriction DNA Cutter

Cut-and-Paste of DNA Using an Artificial Restriction DNA Cutter

DNA manipulations using a completely chemistry-based DNA cutter (ARCUT) have been reviewed. This cutter, recently developed by the authors, is composed of Ce(IV)/EDTA complex and two strands of pseudo-complementary peptide nucleic acid. The site-selective scission proceeds via hydrolysis of targeted...

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Bibliographic Details
Main Author: Makoto Komiyama
Format: Article
Language:English
Published: MDPI AG 2013-02-01
Series:International Journal of Molecular Sciences
Subjects:
site-selective scission
DNA cutter
Ce(IV)/EDTA
PNA
human genome
homologous recombination
restriction enzyme-free manipulation
Online Access:http://www.mdpi.com/1422-0067/14/2/3343

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http://www.mdpi.com/1422-0067/14/2/3343

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