A cDNA Immunization Strategy to Generate Nanobodies against Membrane Proteins in Native Conformation

A cDNA Immunization Strategy to Generate Nanobodies against Membrane Proteins in Native Conformation

Nanobodies (Nbs) are soluble, versatile, single-domain binding modules derived from the VHH variable domain of heavy-chain antibodies naturally occurring in camelids. Nbs hold huge promise as novel therapeutic biologics. Membrane proteins are among the most interesting targets for therapeutic Nbs be...

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Bibliographic Details
Main Authors: Thomas Eden, Stephan Menzel, Janusz Wesolowski, Philine Bergmann, Marion Nissen, Gudrun Dubberke, Fabienne Seyfried, Birte Albrecht, Friedrich Haag, Friedrich Koch-Nolte
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-01-01
Series:Frontiers in Immunology
Subjects:
nanobody
heavy-chain antibody
antibody engineering
cDNA immunization
genetic immunization
membrane proteins
Online Access:http://journal.frontiersin.org/article/10.3389/fimmu.2017.01989/full

Internet

http://journal.frontiersin.org/article/10.3389/fimmu.2017.01989/full

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