Development of novel methodology for the molecular differentiation of Cryptosporidium parvum gp60 subtypes via high resolution melting analysis

Development of novel methodology for the molecular differentiation of Cryptosporidium parvum gp60 subtypes via high resolution melting analysis

Cryptosporidium species subtypes are generally identified via DNA sequencing of the gp60 gene tandem repeat motif region. Due to the immunogenic nature of its glycoprotein products, gp60 is subject to host selective pressures, genetic recombination and evolutionary processes that drive extensive pol...

Full description

Bibliographic Details
Main Authors: Jennifer K. O’ Leary, Liam Blake, Gerard D. Corcoran, Roy D. Sleator, Brigid Lucey
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:MethodsX
Subjects:
Molecular epidemiology
Cryptosporidium
Enteric parasitology
DNA sequencing
Real-time PCR
High resolution melting(HRM) analysis
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016120303770

Internet

http://www.sciencedirect.com/science/article/pii/S2215016120303770

Similar Items