Selection and testing of reference genes for accurate RT-qPCR in rice seedlings under iron toxicity.

Reverse Transcription quantitative PCR (RT-qPCR) is a technique for gene expression profiling with high sensibility and reproducibility. However, to obtain accurate results, it depends on data normalization by using endogenous reference genes whose expression is constitutive or invariable. Although...

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Bibliographic Details
Main Authors: Fabiane Igansi de Castro Dos Santos, Naciele Marini, Railson Schreinert Dos Santos, Bianca Silva Fernandes Hoffman, Marcio Alves-Ferreira, Antonio Costa de Oliveira
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0193418