A simple method to measure CLOCK-BMAL1 DNA binding activity in tissue and cell extracts [version 2; referees: 2 approved, 1 approved with reservations]
The proteins CLOCK and BMAL1 form a heterodimeric transcription factor essential to circadian rhythms in mammals. Daily rhythms of CLOCK-BMAL1 DNA binding activity are known to oscillate with target gene expression in vivo. Here we present a highly sensitive assay that recapitulates native CLOCK-BM...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
F1000 Research Ltd
2017-09-01
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| Series: | F1000Research |
| Subjects: | |
| Online Access: | https://f1000research.com/articles/6-1316/v2 |