Decreased gene expression from T7 promoters may be due to impaired production of active T7 RNA polymerase

<p>Abstract</p> <p>Background</p> <p>Protein expression vectors that utilize the bacteriophage T7 polymerase/promoter system are capable of very high levels of protein production. Frequently, however, expression from these vectors does not reliably achieve optimal level...

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Bibliographic Details
Main Authors: Vethanayagam Joe GG, Flower Ann M
Format: Article
Language:English
Published: BMC 2005-01-01
Series:Microbial Cell Factories
Online Access:http://www.microbialcellfactories.com/content/4/1/3