Decreased gene expression from T7 promoters may be due to impaired production of active T7 RNA polymerase
<p>Abstract</p> <p>Background</p> <p>Protein expression vectors that utilize the bacteriophage T7 polymerase/promoter system are capable of very high levels of protein production. Frequently, however, expression from these vectors does not reliably achieve optimal level...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
BMC
2005-01-01
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Series: | Microbial Cell Factories |
Online Access: | http://www.microbialcellfactories.com/content/4/1/3 |