Isotope derivative assay of microsomal cholesterol 7 α-hydroxylase
A rapid method was developed to measure cholesterol 7 α-hydroxylase activity of hepatic microsomes by the direct determination of the mass of 7 α-hydroxycholesterol formed. The method is based on the quantitative acetylation of the incubation mixture with [-3H]acetic anhydride and the separation of...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1975-03-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752036733X |