Semicontinuous bioreactor production of recombinant butyrylcholinesterase in transgenic rice cell suspension cultures

Semicontinuous bioreactor production of recombinant butyrylcholinesterase in transgenic rice cell suspension cultures

An active and tetrameric form of recombinant butyrylcholinesterase (BChE), a large and complex human enzyme, was produced via semicontinuous operation in a transgenic rice cell suspension culture. After transformation of rice callus and screening of transformants, the cultures were scaled up from cu...

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Bibliographic Details
Main Authors: Jasmine Marie Corbin, Bryce Isao Hashimoto, Kalimuthu eKaruppanan, Zachary Ryan Kyser, Liying eWu, Brian A Roberts, Amy R Noe, Raymond L Rodriguez, Karen Ann McDonald, Somen eNandi
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-03-01
Series:Frontiers in Plant Science
Subjects:
Butyrylcholinesterase
plant cell culture
Semicontinuous culture
Inducible promoter
rice amylase 3D
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00412/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00412/full

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