Identification of Optimal Reference Genes for Normalization of qPCR Analysis during Pepper Fruit Development

Identification of Optimal Reference Genes for Normalization of qPCR Analysis during Pepper Fruit Development

Due to its high sensitivity and reproducibility, quantitative real-time PCR (qPCR) is practiced as a useful research tool for targeted gene expression analysis. For qPCR operations, the normalization with suitable reference genes (RGs) is a crucial step that eventually determines the reliability of...

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Bibliographic Details
Main Authors: Yuan Cheng, Xin Pang, Hongjian Wan, Golam J. Ahammed, Jiahong Yu, Zhuping Yao, Meiying Ruan, Qingjing Ye, Zhimiao Li, Rongqing Wang, Yuejian Yang, Guozhi Zhou
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-06-01
Series:Frontiers in Plant Science
Subjects:
qPCR
reference gene (RG)
normalization
pepper
fruit development
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2017.01128/full

Internet

http://journal.frontiersin.org/article/10.3389/fpls.2017.01128/full

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