Development of a sensitive RT-PCR method for amplifying and sequencing near full-length HCV genotype 1 RNA from patient samples

Development of a sensitive RT-PCR method for amplifying and sequencing near full-length HCV genotype 1 RNA from patient samples

<p>Abstract</p> <p>Background</p> <p>Direct-acting antiviral (DAAs) agents for hepatitis C virus (HCV) span a variety of targets, including proteins encoded by the NS3/4A, NS4B, NS5A, and NS5B genes. Treatment with DAAs has been shown to select variants with sequence ch...

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Bibliographic Details
Main Authors: Zhang Eileen Z, Bartels Doug J, Frantz J Dan, Seepersaud Sheila, Lippke Judith A, Shames Benjamin, Zhou Yi, Lin Chao, Kwong Ann, Kieffer Tara L
Format: Article
Language:English
Published: BMC 2013-02-01
Series:Virology Journal
Subjects:
Hepatitis C Virus (HCV)
Reverse Transcription Polymerase Chain Reaction (RT-PCR)
Genotype
Direct Acting Antiviral Agents (DAA)
Lower Limit of Detection (LLOD)
Quasispecies
Sequencing
Resistance
NS (Non-structural)
NS3/4A
NS4B
NS5A
NS5B
Online Access:http://www.virologyj.com/content/10/1/53

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http://www.virologyj.com/content/10/1/53

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