Optimization of the Production and Purification of Taq Polymerase Enzyme Containing N666E Mutation in its O-Helix Region
Background: The aim of this project was to isolate and purify the highly active recombinant Taq DNA polymerase from the strain of Escherichia coli BL21. This enzyme, with a molecular weight of about 94 kDa, is widely used in polymerase chain reaction (PCR). In PCR, the activity and fidelity of Taq p...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Vesnu Publications
2012-08-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/1897 |