Technical Note: Assay of cell quantity in the fibroblast-populated collagen matrix with a tetrazolium reagent

Determination of cell quantity in three-dimensional culture systems, such as the fibroblast-populated collagen matrix, can be difficult secondary to the abundance of extracellular collagen. The WST-1 assay, which can quantitate cellular processes in monolayer culture, was tested as a measure of rela...

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Bibliographic Details
Main Author: M A Carlson
Format: Article
Language:English
Published: AO Research Institute Davos 2006-09-01
Series:European Cells & Materials
Subjects:
Online Access:http://www.ecmjournal.org/journal/papers/vol012/pdf/v012a05.pdf