Technical Note: Assay of cell quantity in the fibroblast-populated collagen matrix with a tetrazolium reagent
Determination of cell quantity in three-dimensional culture systems, such as the fibroblast-populated collagen matrix, can be difficult secondary to the abundance of extracellular collagen. The WST-1 assay, which can quantitate cellular processes in monolayer culture, was tested as a measure of rela...
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Format: | Article |
Language: | English |
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AO Research Institute Davos
2006-09-01
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Series: | European Cells & Materials |
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Online Access: | http://www.ecmjournal.org/journal/papers/vol012/pdf/v012a05.pdf |