Direct ENIT: An easy and reliable tool for gRNA efficacy verification by tracking induced chromosomal translocation

Direct ENIT: An easy and reliable tool for gRNA efficacy verification by tracking induced chromosomal translocation

CRISPR/Cas systems (Clustered regularly interspaced palindromic repeats / CRISPR-associated) are rapidly becoming a commonplace and popular tool for gene editing in research and clinical contexts. However, the quality of CRISPR/Cas experiments depends heavily on the guide RNA (gRNA) design; therefor...

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Bibliographic Details
Main Authors: Nikolai A. Lomov, Vladimir S. Viushkov, Aleksei V. Zamalutdinov, Maria D. Sboeva, Mikhail A. Rubtsov
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:MethodsX
Subjects:
CRISPR/Cas
Genome editing
Guide RNA testing
Direct PCR
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016120303241

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