Direct ENIT: An easy and reliable tool for gRNA efficacy verification by tracking induced chromosomal translocation
CRISPR/Cas systems (Clustered regularly interspaced palindromic repeats / CRISPR-associated) are rapidly becoming a commonplace and popular tool for gene editing in research and clinical contexts. However, the quality of CRISPR/Cas experiments depends heavily on the guide RNA (gRNA) design; therefor...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2020-01-01
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Series: | MethodsX |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016120303241 |