A protocol for recombinant protein quantification by densitometry

A protocol for recombinant protein quantification by densitometry

Abstract The protein purity is generally checked using SDS‐PAGE, where densitometry could be used to quantify the protein bands. In literature, few studies have been reported using image analysis for the quantification of protein in SDS‐PAGE: that is, imaged with Stain‐Free™ technology. This study p...

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Bibliographic Details
Main Authors: Susana María Alonso Villela, Hazar Kraïem, Balkiss Bouhaouala‐Zahar, Carine Bideaux, César Arturo Aceves Lara, Luc Fillaudeau
Format: Article
Language:English
Published: Wiley 2020-06-01
Series:MicrobiologyOpen
Subjects:
densitometry
image analysis
nanobody production
protein quantitation
SDS‐PAGE
Online Access:https://doi.org/10.1002/mbo3.1027

Internet

https://doi.org/10.1002/mbo3.1027

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