Two-photon scanning microscopy of in vivo sensory responses of cortical neurons genetically encoded with a fluorescent voltage sensor in rat

A fluorescent voltage sensor protein Flare was created from a Kv1.4 potassium channel with YFP situated to report voltage-induced conformational changes in vivo. The RNA virus Sindbis introduced Flare into neurons in the binocular visual crescent in rat. Injection sites were selected based on intrin...

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Bibliographic Details
Main Authors: Kurt F Ahrens, Barbara eHeider, Hanson eLee, Ehud Y Isacoff, Ralph M Siegel
Format: Article
Language:English
Published: Frontiers Media S.A. 2012-03-01
Series:Frontiers in Neural Circuits
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fncir.2012.00015/full