CRISPR/Cas‐based screening of a gene activation library in Saccharomyces cerevisiae identifies a crucial role of OLE1 in thermotolerance

Summary CRISPR/Cas‐based (clustered regularly interspaced short palindromic repeats/CRISPR‐associated) screening has been proved to be an efficient method to study functional genomics from yeast to human. In this study, we report the development of a focused CRISPR/Cas‐based gene activation library...

Full description

Bibliographic Details
Main Authors: Pengsong Li, Xiaofen Fu, Lei Zhang, Shizhong Li
Format: Article
Language:English
Published: Wiley 2019-11-01
Series:Microbial Biotechnology
Online Access:https://doi.org/10.1111/1751-7915.13333