ZAK negatively regulates RhoGDIβ-induced Rac1-mediated hypertrophic growth and cell migration

• Main Authors: Chang I-Chang, Liu Kuan-Yu, Yang Li-Chiu, Huang Chih-Yang, Liao Pao-Hsin, Chou Janet, Chou Ming-Yung, Lin Wei-Wen, Yang Jaw-Ji
• Format: Article
• Language: English
• Published: BMC 2009-06-01
• Series: Journal of Biomedical Science
• Online Access: http://www.jbiomedsci.com/content/16/1/56

<p>Abstract</p> <p>RhoGDIβ, a Rho GDP dissociation inhibitor, induced hypertrophic growth and cell migration in a cultured cardiomyoblast cell line, H9c2. We demonstrated that RhoGDIβ plays a previously undefined role in regulating Rac1 expression through transcription to induce hypertrophic growth and cell migration and that these functions are blocked by the expression of a dominant-negative form of Rac1. We also demonstrated that knockdown of RhoGDIβ expression by RNA interference blocked RhoGDIβ-induced Rac1 expression and cell migration. We demonstrated that the co-expression of ZAK and RhoGDIβ in cells resulted in an inhibition in the activity of ZAK to induce ANF expression. Knockdown of ZAK expression in ZAK-RhoGDIβ-expressing cells by ZAK-specific RNA interference restored the activities of RhoGDIβ.</p>