EXPRESSION OF RECOMBINANT L-PHENYLALANINE AMMONIA-LYASE IN ESCHERICHIA COLI

EXPRESSION OF RECOMBINANT L-PHENYLALANINE AMMONIA-LYASE IN ESCHERICHIA COLI

The pal gene coding for L-phenylalanine ammonia-lyase of Rhodosporidium toruloides (GenBank entry no. X12702.1) with optimized sequence was cloned into an expressing vector pET28a. Three parameters of expression (inductor type, duration, and temperature of induction) were optimized, which resulted i...

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Bibliographic Details
Main Authors: Babich O.O., Dyshlyuk L., Milent`eva I.S.
Format: Article
Language:English
Published: Kemerovo State University 2013-12-01
Series:Foods and Raw Materials
Subjects:
L-phenylalanine ammonia-lyase
cloning
expression
recombinant protein
induction
L-phenylalanine
phenylketonuria
Online Access:http://jfrm.ru/?page=archive&jrn=1&article=7

Internet

http://jfrm.ru/?page=archive&jrn=1&article=7

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