A novel real-time PCR assay of microRNAs using S-Poly(T), a specific oligo(dT) reverse transcription primer with excellent sensitivity and specificity.

A novel real-time PCR assay of microRNAs using S-Poly(T), a specific oligo(dT) reverse transcription primer with excellent sensitivity and specificity.

BACKGROUND: MicroRNAs (miRNAs) are small, non-coding RNAs capable of postranscriptionally regulating gene expression. Accurate expression profiling is crucial for understanding the biological roles of miRNAs, and exploring them as biomarkers of diseases. METHODOLOGY/PRINCIPAL FINDINGS: A novel, high...

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Bibliographic Details
Main Authors: Kang Kang, Xiaoying Zhang, Hongtao Liu, Zhiwei Wang, Jiasheng Zhong, Zhenting Huang, Xiao Peng, Yan Zeng, Yuna Wang, Yi Yang, Jun Luo, Deming Gou
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3496722?pdf=render

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http://europepmc.org/articles/PMC3496722?pdf=render

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