Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins

<p>Abstract</p> <p>Background</p> <p>Expression of higher eukaryotic genes as soluble, stable recombinant proteins is still a bottleneck step in biochemical and structural studies of novel proteins today. Correct identification of stable domains/fragments within the ope...

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Bibliographic Details
Main Authors: Luan Chi-Hao, Qiu Shihong, Chen Yunjia, Luo Ming
Format: Article
Language:English
Published: BMC 2007-07-01
Series:BMC Biotechnology
Online Access:http://www.biomedcentral.com/1472-6750/7/45