Light-Controlled Affinity Purification of Protein Complexes Exemplified by the Resting ZAP70 Interactome

Light-Controlled Affinity Purification of Protein Complexes Exemplified by the Resting ZAP70 Interactome

Multiprotein complexes control the behavior of cells, such as of lymphocytes of the immune system. Methods to affinity purify protein complexes and to determine their interactome by mass spectrometry are thus widely used. One drawback of these methods is the presence of false positives. In fact, the...

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Bibliographic Details
Main Authors: Maximilian Hörner, Julian Eble, O. Sascha Yousefi, Jennifer Schwarz, Bettina Warscheid, Wilfried Weber, Wolfgang W. A. Schamel
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-02-01
Series:Frontiers in Immunology
Subjects:
optogenetics
affinity purification
phytochrome
ZAP70
protein-protein interaction
mass spectrometry
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2019.00226/full

Internet

https://www.frontiersin.org/article/10.3389/fimmu.2019.00226/full

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