Taking advantage of a high-throughput flow cytometer for the implementation of an ADCC assay for regulatory compliance

• Main Authors: Rosa Camacho-Sandoval, Alexis Jiménez-Uribe, Alejandra V. Tenorio-Calvo, Carlos A. López-Morales, Leslie Muñoz-García, Alejandra Montes-Luna, Héctor Leonardo García-Xolalpa, Marco Velasco-Velázquez, Lenin Pavón, Sonia Mayra Pérez-Tapia, Emilio Medina-Rivero
• Format: Article
• Language: English
• Published: Elsevier 2020-06-01
• Series: Biotechnology Reports
• Subjects: ADCC; High-throughput flow cytometry; Validation bioassay
• Online Access: http://www.sciencedirect.com/science/article/pii/S2215017X19305181
• ISSN: 2215-017X

Technological advances allowed the development of high-throughput instruments such as IntelliCyt iQue Screener PLUS®. Here, we took advantage of this technology to transfer a previously validated cytotoxicity assay. The evaluated parameters were cell permeability, caspase activation and phosphatidyl serine exposure. The assay was accurate (r2 = 0.90), precise (%CV ≤ 18.90) and specific. These results showed that this technology is suitable to be used in control quality environments. In addition, the automation provided a faster acquisition and analysis of data with precise and accurate results. This application could be implemented to evaluate another in vitro mechanism of action of different biotherapeutics.