Visualization of Ceramide-Associated Proteins in Ceramide-Rich Platforms Using a Cross-Linkable Ceramide Analog and Proximity Ligation Assays With Anti-ceramide Antibody

Visualization of Ceramide-Associated Proteins in Ceramide-Rich Platforms Using a Cross-Linkable Ceramide Analog and Proximity Ligation Assays With Anti-ceramide Antibody

Ceramide-rich platforms (CRPs) mediate association of proteins with the sphingolipid ceramide and may regulate protein interaction in membrane contact sites to the cytoskeleton, organelles, and infectious pathogens. However, visualization of ceramide association to proteins is one of the greatest ch...

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Bibliographic Details
Main Authors: Xue Jiang, Zhihui Zhu, Haiyan Qin, Priyanka Tripathi, Liansheng Zhong, Ahmed Elsherbini, Sanjib Karki, Simone M. Crivelli, Wenbo Zhi, Guanghu Wang, Stefanka D. Spassieva, Erhard Bieberich
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-08-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
ceramide
proximity ligation assay
cross-link
lipid raft
microtubules
mitochondria
Online Access:https://www.frontiersin.org/article/10.3389/fcell.2019.00166/full

Internet

https://www.frontiersin.org/article/10.3389/fcell.2019.00166/full

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