Size of gene specific inverted repeat--dependent gene deletion In Saccharomyces cerevisiae.

We describe here an approach for rapidly producing scar-free and precise gene deletions in S. cerevisiae with high efficiency. Preparation of the disruption gene cassette in this approach was simply performed by overlap extension-PCR of an invert repeat of a partial or complete sequence of the targe...

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Bibliographic Details
Main Authors: Chanyuen Lim, Annette Lin Luhe, Crystal Tear Jingying, Balaji Balagurunathan, Jinchuan Wu, Hua Zhao
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3748122?pdf=render