Development of a pyrosequencing assay for the typing of alphaherpesviruses
Identification of herpesvirus in biological material is usually carried out by real-time PCR. With the aim to classify the strain of virus identified, real-time PCR must be often supported by time-consuming capillary electrophoresis sequencing analysis. Here we provide a protocol for the rapid and r...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2015-01-01
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Series: | MethodsX |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016115000023 |