Development of a pyrosequencing assay for the typing of alphaherpesviruses

Identification of herpesvirus in biological material is usually carried out by real-time PCR. With the aim to classify the strain of virus identified, real-time PCR must be often supported by time-consuming capillary electrophoresis sequencing analysis. Here we provide a protocol for the rapid and r...

Full description

Bibliographic Details
Main Authors: G. Fusco, M.G. Amoroso, N. Gesualdi Montesano, M. Viscardi
Format: Article
Language:English
Published: Elsevier 2015-01-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016115000023