Engineering of CRISPR-Cas12b for human genome editing
The Cas12b family of CRISPR nucleases has been underutilized in mammalian cells due to the high temperature requirement of known members. Here the authors engineer BhCas12b to overcome this limitation for robust and specific genome editing applications in human cells.
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2019-01-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-018-08224-4 |