EndoBind detects endogenous protein-protein interactions in real time

EndoBind detects endogenous protein-protein interactions in real time

Bill et al describe two high-throughput methods to detect protein-protein interactions in cells in real-time using the split-NanoLuciferase-complementation system. They demonstrate the methods can detect exogenously (RT-bind) or endogenously (EndoBind) expressed proteins, respectively.

Bibliographic Details
Main Authors: Anke Bill, Sheryll Espinola, Daniel Guthy, Jacob R. Haling, Mylene Lanter, Min Lu, Anthony Marelli, Angelica Mendiola, Loren Miraglia, Brandon L. Taylor, Leonardo Vargas, Anthony P. Orth, Frederick J. King
Format: Article
Language:English
Published: Nature Publishing Group 2021-09-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-021-02600-5

Internet

https://doi.org/10.1038/s42003-021-02600-5

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