Using Next-Generation Sequencing and Cross-Species Amplification in the Genus Pseudopungtungia

Using Next-Generation Sequencing and Cross-Species Amplification in the Genus Pseudopungtungia

Nuclear microsatellite markers for Pungtungia herzi were developed using a combination of next-generation sequencing and Sanger sequencing. One hundred primer sets in the flanking region of dinucleotide and trinucleotide repeat motifs were designed and tested for efficiency in polymerase chain react...

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Bibliographic Details
Main Authors: Myounghai Kwak, Young-Eun Yun, Jeong-Nam Yu, Sang Ki Kim, Ui Wook Hwang
Format: Article
Language:English
Published: MDPI AG 2013-10-01
Series:International Journal of Molecular Sciences
Subjects:
microsatellite
Pungtungia herzi
Pseudopungtungia
cross-species amplification
next-generation sequencing
Online Access:http://www.mdpi.com/1422-0067/14/10/19923

Internet

http://www.mdpi.com/1422-0067/14/10/19923

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