Live-cell nanoscopy enabled with transient labeling and the control of fluorophore blinking

Live-cell nanoscopy enabled with transient labeling and the control of fluorophore blinking

Live-cell super-resolution of proteins labeled with genetically encoded fluorescent tags is a challenging task because of the imperfect labeling and the inevitable deterioration of the signal in the course of the experiment. Incomplete maturation of the covalently attached fluorescent tags, ineffici...

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Main Authors: Mishin Alexander, Perfilov Maxim, Gavrikov Alexey, Mamontova Anastasia, Bogdanov Alexey, Lukyanov Konstantin
Format: Article
Language:English
Published: EDP Sciences 2018-01-01
Series:EPJ Web of Conferences
Online Access:https://doi.org/10.1051/epjconf/201819003008
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spelling doaj-e6500ab1427249f6a887cda24fec70792021-08-02T08:13:12ZengEDP SciencesEPJ Web of Conferences2100-014X2018-01-011900300810.1051/epjconf/201819003008epjconf_hbsm2018_03008Live-cell nanoscopy enabled with transient labeling and the control of fluorophore blinkingMishin AlexanderPerfilov MaximGavrikov AlexeyMamontova AnastasiaBogdanov AlexeyLukyanov KonstantinLive-cell super-resolution of proteins labeled with genetically encoded fluorescent tags is a challenging task because of the imperfect labeling and the inevitable deterioration of the signal in the course of the experiment. Incomplete maturation of the covalently attached fluorescent tags, inefficient photoconversion, and photobleaching further complicate prolonged live-cell nanoscopy. We have implemented two strategies for lowering the photodamage: ensuring the dynamic replacement of damaged molecules and establishing conditions for the robust intrinsic blinking of the tags at lower illumination powers.https://doi.org/10.1051/epjconf/201819003008
collection DOAJ
language English
format Article
sources DOAJ
author Mishin Alexander
Perfilov Maxim
Gavrikov Alexey
Mamontova Anastasia
Bogdanov Alexey
Lukyanov Konstantin
spellingShingle Mishin Alexander
Perfilov Maxim
Gavrikov Alexey
Mamontova Anastasia
Bogdanov Alexey
Lukyanov Konstantin
Live-cell nanoscopy enabled with transient labeling and the control of fluorophore blinking
EPJ Web of Conferences
author_facet Mishin Alexander
Perfilov Maxim
Gavrikov Alexey
Mamontova Anastasia
Bogdanov Alexey
Lukyanov Konstantin
author_sort Mishin Alexander
title Live-cell nanoscopy enabled with transient labeling and the control of fluorophore blinking
title_short Live-cell nanoscopy enabled with transient labeling and the control of fluorophore blinking
title_full Live-cell nanoscopy enabled with transient labeling and the control of fluorophore blinking
title_fullStr Live-cell nanoscopy enabled with transient labeling and the control of fluorophore blinking
title_full_unstemmed Live-cell nanoscopy enabled with transient labeling and the control of fluorophore blinking
title_sort live-cell nanoscopy enabled with transient labeling and the control of fluorophore blinking
publisher EDP Sciences
series EPJ Web of Conferences
issn 2100-014X
publishDate 2018-01-01
description Live-cell super-resolution of proteins labeled with genetically encoded fluorescent tags is a challenging task because of the imperfect labeling and the inevitable deterioration of the signal in the course of the experiment. Incomplete maturation of the covalently attached fluorescent tags, inefficient photoconversion, and photobleaching further complicate prolonged live-cell nanoscopy. We have implemented two strategies for lowering the photodamage: ensuring the dynamic replacement of damaged molecules and establishing conditions for the robust intrinsic blinking of the tags at lower illumination powers.
url https://doi.org/10.1051/epjconf/201819003008
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AT gavrikovalexey livecellnanoscopyenabledwithtransientlabelingandthecontroloffluorophoreblinking
AT mamontovaanastasia livecellnanoscopyenabledwithtransientlabelingandthecontroloffluorophoreblinking
AT bogdanovalexey livecellnanoscopyenabledwithtransientlabelingandthecontroloffluorophoreblinking
AT lukyanovkonstantin livecellnanoscopyenabledwithtransientlabelingandthecontroloffluorophoreblinking
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