Live-cell nanoscopy enabled with transient labeling and the control of fluorophore blinking

Live-cell super-resolution of proteins labeled with genetically encoded fluorescent tags is a challenging task because of the imperfect labeling and the inevitable deterioration of the signal in the course of the experiment. Incomplete maturation of the covalently attached fluorescent tags, ineffici...

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Bibliographic Details
Main Authors: Mishin Alexander, Perfilov Maxim, Gavrikov Alexey, Mamontova Anastasia, Bogdanov Alexey, Lukyanov Konstantin
Format: Article
Language:English
Published: EDP Sciences 2018-01-01
Series:EPJ Web of Conferences
Online Access:https://doi.org/10.1051/epjconf/201819003008