Toxin-Activating Stapled Peptides Discovered by Structural Analysis Were Identified as New Therapeutic Candidates that Trigger Antibacterial Activity against <i>Mycobacterium tuberculosis </i>in the <i>Mycobacterium smegmatis</i> Model

• Main Authors: Sung-Min Kang, Heejo Moon, Sang-Woo Han, Byeong Wook Kim, Do-Hee Kim, Byeong Moon Kim, Bong-Jin Lee
• Format: Article
• Language: English
• Published: MDPI AG 2021-03-01
• Series: Microorganisms
• Subjects: toxin-antitoxin system; structure-based drug discovery; antimicrobial candidate; stapled peptide
• Online Access: https://www.mdpi.com/2076-2607/9/3/568
• ISSN: 2076-2607

The structure-function relationships of toxin-antitoxin (TA) systems from <i>Mycobacterium tuberculosis</i> have prompted the development of novel and effective antimicrobial agents that selectively target this organism. The artificial activation of toxins by peptide inhibitors can lead to the growth arrest and eventual death of bacterial cells. Optimizing candidate peptides by hydrocarbon α-helix stapling based on structural information from the VapBC TA system and in vitro systematic validation led to <b>V26-SP-8</b>, a VapC26 activator of <i>M. tuberculosis</i>. This compound exhibited highly enhanced activity and cell permeability owing to the stabilizing helical propensity of the peptide. These characteristics will increase its efficacy against multidrug-resistant tuberculosis and extensively drug-resistant tuberculosis. Similar approaches utilizing structural and biochemical information for new antibiotic targets opens a new era for developing TB therapies.