Engineering of gentiopicroside-producing yeast strain using low-energy ion implantation mediated synthetic biology

To obtain an alternative source for the production of gentiopicroside, here genomic DNA segments of the medicinal plant Gentiana macrophylla were randomly transferred into Hansenula polymorpha by 25 KeV nitrogen ions (N+) at a dose of 2.5 × 1016 ions/cm2 under vacuum pressure of 1 × 10−3 Pa. To scre...

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Bibliographic Details
Main Authors: Ting Wang, Weidong Qian, Yunfang Fu, Changlong Cai, Peihong Mao
Format: Article
Language:English
Published: Taylor & Francis Group 2016-07-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
Online Access:http://dx.doi.org/10.1080/13102818.2016.1175320