Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1
Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specifici...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2000-04-01
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| Series: | Memórias do Instituto Oswaldo Cruz. |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200015 |