Enhancement of a protocol purifying T1 lipase through molecular approach

Enhancement of a protocol purifying T1 lipase through molecular approach

T1 Lipase is a thermostable secretary protein of Geobacillus zalihae strain previously expressed in a prokaryotic system and purified using three-step purification: affinity 1, affinity 2, and ion exchange chromatography (IEX). This approach is time consuming and offers low purity and recovery yield...

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Bibliographic Details
Main Authors: Che Haznie Ayu Che Hussian, Raja Noor Zaliha Raja Abd. Rahman, Adam Leow Thean Chor, Abu Bakar Salleh, Mohd Shukuri Mohamad Ali
Format: Article
Language:English
Published: PeerJ Inc. 2018-11-01
Series:PeerJ
Subjects:
Isoelectric point
E. coli
Lipase
Purifications
Site-directed mutagenesis
Online Access:https://peerj.com/articles/5833.pdf

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https://peerj.com/articles/5833.pdf

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