Quantitative analysis of cholesterol in 5 to 20 μl of plasma
A gas–liquid chromatographic micromethod for quantitation of cholesterol in 20 μl of plasma was developed using 5α-cholestane as an internal standard, saponification with tetramethylammonium hydroxide–isopropanol, and extraction with tetrachloroethylene–methyl butyrate. Cholesterol levels in plasma...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
1974-05-01
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Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520368097 |