Quantitative analysis of cholesterol in 5 to 20 μl of plasma

A gas–liquid chromatographic micromethod for quantitation of cholesterol in 20 μl of plasma was developed using 5α-cholestane as an internal standard, saponification with tetramethylammonium hydroxide–isopropanol, and extraction with tetrachloroethylene–methyl butyrate. Cholesterol levels in plasma...

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Bibliographic Details
Main Authors: T.T. Ishikawa, J. MacGee, J.A. Morrison, C.J. Glueck
Format: Article
Language:English
Published: Elsevier 1974-05-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520368097