Hypothermic Storage of Periportal and Perivenous Rat Hepatocytes

High yields of intact parenchymal cells are produced by the two-step Digitonin-collagenase perfusion of whole liver, and it has gained wide acceptance for biochemical and cellular analyses of zonal hepatocytes. The development reached by this methodology is in contrast to the time-limited use of the...

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Bibliographic Details
Main Authors: Edgardo Elvio Guibert, María Gabriela Mediavilla, María Eugenia Mamprin, Joaquín Valentín Rodríguez
Format: Article
Language:English
Published: SAGE Publishing 1998-07-01
Series:Cell Transplantation
Online Access:https://doi.org/10.1177/096368979800700402