Fluorescent derivatization of a protease antigen to track antigen uptake and processing in human cell lines

<p>Abstract</p> <p>Background</p> <p>We have devised a simple and efficient fluorescence-based method to track antigen uptake and processing in human B lymphoblastoid cells (B-LCL). Fluorescein labelled subtilisin was used to optimize antigen uptake conditions and ident...

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Bibliographic Details
Main Authors: Collier Katherine D, Wong David L, Patil Namrata S, McDonald Hugh C
Format: Article
Language:English
Published: BMC 2004-06-01
Series:BMC Immunology
Online Access:http://www.biomedcentral.com/1471-2172/5/12