Imaging Live Cells at the Nanometer-Scale with Single-Molecule Microscopy: Obstacles and Achievements in Experiment Optimization for Microbiology

Single-molecule fluorescence microscopy enables biological investigations inside living cells to achieve millisecond- and nanometer-scale resolution. Although single-molecule-based methods are becoming increasingly accessible to non-experts, optimizing new single-molecule experiments can be challen...

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Bibliographic Details
Main Authors: Beth L. Haas, Jyl S. Matson, Victor J. DiRita, Julie S. Biteen
Format: Article
Language:English
Published: MDPI AG 2014-08-01
Series:Molecules
Subjects:
Online Access:http://www.mdpi.com/1420-3049/19/8/12116