Purification and properties of carotene 15,15′-dioxygenase of rabbit intestine
Carotene 15,15′-dioxygenase, which oxidizes carotenoids to retinal, has been purified up to 200-fold from rabbit intestine by ammonium sulfate fractionation, heat treatment, and acetone precipitation. With β-apo-10′-carotenol as the substrate, the purified enzyme has a pH optimum of 7.8, a Km, of 6....
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
1972-07-01
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Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520393810 |