Purification and properties of carotene 15,15′-dioxygenase of rabbit intestine

Carotene 15,15′-dioxygenase, which oxidizes carotenoids to retinal, has been purified up to 200-fold from rabbit intestine by ammonium sulfate fractionation, heat treatment, and acetone precipitation. With β-apo-10′-carotenol as the substrate, the purified enzyme has a pH optimum of 7.8, a Km, of 6....

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Bibliographic Details
Main Authors: M.R. Lakshmanan, Hansa Chansang, James A. Olson
Format: Article
Language:English
Published: Elsevier 1972-07-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520393810